ILLUSTRATED USE OF A SINGLE INSTI™ TEST KIT
The INSTI™ HIV-1/HIV-2 Rapid Antibody Test is a rapid in vitro qualitative test for the detection of antibodies to Human Immunodeficiency Virus Type 1 and Type 2 in human whole blood, fingerstick blood, serum or plasma. The test is intended for use by trained personnel in medical facilities, clinical laboratories, emergency care situations, and physicians' offices as a screening assay capable of providing test results in as little as 60 seconds. The assay is packaged as a kit containing INSTI™ Membrane Units, Sample Diluent, Color Developer and Clarifying Solution, and is available in point-of-care use packaging, or packaging suitable for laboratory use.
Collect 50µl of fingerstick blood, venous whole blood, serum or plasma and add bottle number 1, Sample Diluent, re-cap and invert 3-4 times. |
1 Pour the entire contents of bottle number 1 into the center of the Membrane Unit well. HIV antibodies, if present, are captured at the test spot by proteins on the membrane. |
2 Add the entire contents of bottle number 2, Colour Developer, into the center of the Membrane Unit well to generate a blue control spot and a second spot if HIV-1/HIV-2 antibodies are present. |
3 Add the entire contents of bottle number 3, Clarifying Solution into center of the Membrane Unit well to reduce background colour and produce more distinct test and control spots. The control spot will appear only if human blood or blood component is present. |
